Leja slides can be extremely useful in the investigation of semen of aquatic animals. Longevity of cells can be a major concern when testing samples. The disposable Leja counting chambers can assist you in the sample evaluation.
In order to perform a reliable cocentration analysis, Leja recommend to perform an accurant dilution of a part of the sample to local protocol. Do not dilute the entire sample! We forward you to the following documents to perform the analysis:
Leja advises to use 20 micron deep chambers for most aquaculture evaluation:
- SC-20-01-02-B: 2 chamber slide - sample volume ~6µl
- SC-20-01-04-B: 4 chamber slide - sample volume ~3µl
- SC-20-01-08-B: 8 chamber slide - sample volume ~2µl (please note: flushing technique is very difficult in 8 chamber slide)
Leja offers starter-kits, these kits come with all you need to start working with Leja slides within 10 minutes in case you do not work with a CASA system:
A CASA system is highly recommended when you desire to analyse the motility of semen. Leja recommends the "Flush technique" when filling the chambers in order to get optimal result:
|Prepare a Leja slide on the object table of your microscope. Prepare the CASA system and prepare all other necessary equipment so that you can handle quickly.
|Apply a tiny amount of milt in the opening of the first chamber. Use your microscope to focus on the semen cells in the milt. Now move the object table in such a way that the ocular is directly above the approximate middle of the counting chamber.
Steadily apply enough activation medium to fill the entire chamber in one swift movement. Check the focus of the microscope simultaneously while the milt is dispersed in the chamber. As soon as the drift of the cells has stopped, start the CASA analysis and record the results.